Single-cell gene expression profiles of cells from primary colorectal cancer and adjacent normal tissue [scRNA-Seq]

Colorectal cancer (CRC) is the third malignancy worldwide. RAS mutant CRC has a worse prognosis and resistant to immune therapies. Current research on the tumor microenvironment of RAS-mutant CRC remains limited, with few studies systematically characterizing its cellular composition or functional dynamics. A total of 36 clinical surgical samples (tumors and paired adjacent normal tissues) from 18 patients with primary CRC were collected for single-cell transcriptome sequencing.At the same time, each patient underwent genome sequencing, and 18 patients were divided into RAS gene mutation group (n=10) and wild-type group (n=8). Therefore, revealing the functional cell types and mechanism of immune evasion in RAS mutant CRC by scRNA-seq may contribute to discover new therapeutic targets.