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eIF4G2-mediated Translation Initiation of Histone Modifiers is Essential for Intestinal Stem Cell Maintenance and Differentiation [RNA-seq]

GSE294512 Mus musculus (mouse) RNA-seq (gene expression) 92 samples Submitted 2026/04/30 Platform GPL24247Platform GPL34290
At a glance

RNA-seq (gene expression) · mouse · 92 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
92 samples: 21 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
eIF4G2 (also known as NAT1, p97, and DAP5) is an evolutionally conserved protein homologous to the c-terminal two thirds portion of eukaryotic translation initiation factor (eIF) 4G. Despite its abundant and ubiquitous expression, physiological and pathological functions of eIF4G2 are poorly understood. Here we show that acute inactivation of eIF4G2 in adult mice results in rapid weight loss with abnormalities in multiple organs.
Read the full summary ↓
In this study, we show that eIF4G2 is critical for translation of key histone modification proteins involved in maintenance and differentiation of intestinal stem cells. Our study underscores the importance of eIF4G2-mediated translation in multicellular organisms.
This dataset
Open GSE294512 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE294512_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1250430 and SRA study SRP578610. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
21 conditions, mostly with about 4 replicates each
Homozygous, Villi, 3 months, ×6 Spheroid, flox/flox, ENR, DMSO, ×6 Spheroid, flox/flox, ENR, 4-OHT, ×6 Heterozygous, Crypt, ×5 Homozygous, Villi, ×5 Heterozygous, Villi, ×5 Spheroid, flox/+, ENR, DMSO, ×5 Spheroid, flox/+, ENR, 4-OHT, ×5 +13 more

Supports a case/control comparison: 12 samples read as cases, 18 as controls.

How we read this: 21 replicated groups read from 92 sample titles; they account for 92 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 52 more — browse all 92 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.