Effects of propofol on human brain organoids at different maturational stages

Human brain organoids were derived from the H1 human embryonic stem cell line and exposed at different developmental stages to propofol (50 µM) for 3 days in vitro (div). Human brain organoids were investigated in four experimental groups at two developmental stages, 60 div and 90 div, including mock-treated groups as control and propofol-treated groups (from 47-50 div or 77-80 div, n=5 each). Our results suggest that propofol affects synapse-associated gene expression and neuronal activity differently at specific developmental stages.