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SHROOM3 Deficiency aggravates Adriamycin-induced nephropathy accompanied by Focal Adhesion disassembly and stress fiber disorganization

GSE294734 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/04/17 Platform GPL24676
Summary
This RNA-sequencing study investigates the role of SHROOM3 in podocyte function and its contribution to renal pathophysiology. Podocytes are highly specialized epithelial cells critical for glomerular filtration barrier integrity. The study explores how SHROOM3 deficiency affects podocyte cytoskeletal architecture and focal adhesion dynamics in the context of Adriamycin-induced nephropathy, a widely used experimental model of podocyte injury and proteinuric kidney disease. We performed transcriptome analysis using RNA-sequencing on human podocyte cell lines, comparing Control (n=4) and SHROOM3 knockdown (KD) podocytes (n=4). Our objective was to identify the molecular mechanisms and signaling pathways through which SHROOM3 regulates podocyte cytoskeletal organization, with particular focus on stress fiber formation and focal adhesion assembly/disassembly. The dataset provides comprehensive gene expression profiles revealing how SHROOM3 deficiency exacerbates podocyte injury, disrupts actin cytoskeleton dynamics, and impairs focal adhesion stability during nephropathy progression.
Published in
SHROOM3 Deficiency Aggravates Adriamycin-Induced Nephropathy Accompanied by Focal Adhesion Disassembly and Stress Fiber Disorganization
Xu LN, Sun YY, Tan YF et al. · Cells 2025 · PMID 40558522 · doi:10.3390/cells14120895
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Direct links to NCBI, no account and no request form: the whole study as GSE294734_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1251520 and SRA study SRP578989. Searching any of these in the dataset finder brings you back here.

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