The effect of UV irradiation on the distribution of RNAPII NTD and DIS3

It is well established that exposure to UV light alters the distribution of RNA polymerase II (RNAPII), but the impact of UV irradiation on the genomic distribution of DIS3 has not been investigated. We generated ChIP-seq profiles of DIS3 in U2OS cells that express a HA-tagged DIS3 protein (DIS3FLAGHA). We analyzed the total occupancy of RNAPII using an antibody against the N-terminal domain of RPB1 (NTD). Average RNAPII occupancy at transcription start sites (TSSs) was significantly decrease 3 hours after UV irradiation, consistent with previous reports, and was notably recovered 8 hours after irradiation. Upon UV irradiation, DIS3 exhibited a redistribution that closely aligned with the changes observed in RNAPII. This similarity, was particularly pronounced in highly expressed genes. Our results suggests that DIS3 is associated with the transcription machinery and can act locally at the transcription site during elongation.