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Vaccination against Onchocerca volvulus induces IgG-mediated protective immunity dependent on neutrophils and complement

GSE295728 Mus musculus (mouse) RNA-seq (gene expression) 47 samples Submitted 2025/09/10 Platform GPL30172
At a glance

RNA-seq (gene expression) · mouse · 47 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
47 samples: 8 conditions, mostly with about 6 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Vaccination against Onchocerca volvulus induces IgG-mediated protective immunity dependent on neutrophils and complementNPJ vaccines · 2025
Summary (written by the submitters)
Onchocerciasis remains a significant cause of morbidity and economic loss in sub-Saharan Africa. Despite the existence of effective therapeutics, a prophylactic vaccine targeting the etiologic agent, Onchocerca volvulus, is needed to control ongoing disease and transmission. Mice were vaccinated against O. volvulus with a fusion of the recombinant antigens Ov-103 and Ov-RAL-2 (Ov-FUS-1) with Advax-CpG adjuvant.
Read the full summary ↓
Immunized mice developed protective immunity with the killing of third-stage larvae (L3) within 36 hours of challenge infection. IgG from immunized mice passively transferred protective immunity to naïve mice, indicating that antigen-specific IgG mediated parasite elimination. Neutrophils were the most abundant subset of immune cells recruited to the parasite microenvironment in vivo, and their depletion resulted in the total loss of immune-mediated larval killing. Analysis of neutrophil gene expression revealed that both vaccination and the presence of O. volvulus larvae were capable of modulating neutrophil transcriptional activity. The mechanism by which antigen-specific IgG and neutrophils collaborated to kill L3 was independent of Fcγ receptors. However, the elimination of complement component C3 prevented vaccine-induced protection, which suggests these components may interact through the complement system. This study describes a vaccine-induced mechanism of protective immunity against O. volvulus L3 dependent on IgG, neutrophils, and complement, highlighting an effective collaboration between the innate and adaptive arms of the immune system to control O. volvulus infection.
Published in
Vaccination against Onchocerca volvulus induces IgG-mediated protective immunity dependent on neutrophils and complement
Ryan NM, Hess JA, Nakhale M et al. · NPJ vaccines 2025 · PMID 41125576 · doi:10.1038/s41541-025-01267-x
This dataset
Open GSE295728 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE295728_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1256276 and SRA study SRP581962. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41125576) ↗
Study design
8 conditions, mostly with about 6 replicates each
mouse neutrophil, 36h larvae imm, ×7 mouse neutrophil, 18h larvae imm, ×6 mouse neutrophil, 18h larvae ctrl, ×6 mouse neutrophil, 18h no-larvae imm, ×6 mouse neutrophil, 36h larvae ctrl, ×6 mouse neutrophil, 36h no-larvae ctrl, ×6 mouse neutrophil, 18h no-larvae ctrl, ×5 mouse neutrophil, 36h no-larvae imm, ×5

Supports a between-group comparison across 47 samples.

How we read this: 8 replicated groups read from 47 sample titles; they account for 47 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 7 more — browse all 47 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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