GEO series

Improved Small Interfering RNA Therapy Targeting the Long, Non-Coding RNA SMILR For Therapeutic Intervention in Coronary Artery Bypass Graft Failure

GSE296137 Homo sapiens (human) RNA-seq (gene expression) 20 samples Submitted 2026/05/01 Platform GPL24676

At a glance

RNA-seq (gene expression) · human · 20 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Samples: 20 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Small Interfering RNA Therapy Targeting the Long Noncoding RNA SMILR for Therapeutic Intervention in Coronary Artery Bypass Graft FailureJACC. Basic to translational science · 2025

Summary (written by the submitters)

Coronary artery bypass graft (CABG) surgery remains the gold standard of care to prevent myocardial ischaemia in patients with advanced atherosclerosis, however long-term graft patency remains a considerable and long-standing problem. Excessive vascular smooth muscle cell (vSMC) proliferation in the grafted tissue is recognised as central to late CABG failure.

Read the full summary ↓

We previously showed that siRNA-mediated silencing of SMILR, a human-specific SMC-enriched lncRNA, blocks vSMC proliferation, suggesting that targeting SMILR expression could be a novel way to prevent neointima formation, and thus CABG failure. Here, we describe the design and synthesis of a library of 76 chemically-enhanced SMILR-targeting siRNA. From this library, we identify a lead siRNA, BHF7, which demonstrates potent and reproducible silencing of SMILR expression and which robustly blocks vascular smooth muscle cell proliferation, both in vitro and up to 14 days in the ex vivo human saphenous vein model. We further demonstrate using RNA-sequencing that BHF7 down-regulates the expression of genes associated with proliferation and does not induce the expression of interferon or apoptosis genes, suggesting it has an acceptable safety profile both on and off-target. Finally, we performed TUNEL staining and measured the levels of cleaved caspase-3 after BHF7 treatment which demonstrated that BHF7 does not induce a cytotoxic response either in vitro or ex vivo. Collectively, this data represents a pre-clinical package into the function and specificity of BHF7 which warrants further investigation into the possibility of utilising BHF7 as a novel, ex vivo RNA therapeutic for the prevention of CABG failure.

Published in

Small Interfering RNA Therapy Targeting the Long Noncoding RNA SMILR for Therapeutic Intervention in Coronary Artery Bypass Graft Failure

Brown SD, Malinowska AL, Bennett M et al. · JACC. Basic to translational science 2025 · PMID 40928446 · doi:10.1016/j.jacbts.2025.101364

This dataset

Open GSE296137 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE296137_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1257846 and SRA study SRP582638. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40928446) ↗

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 20 more — browse all 20 samples with per-sample file links →

Datasets on a similar topic

GSE280530 Systematic identification and characterization of high efficiency Cas9 guide RNAs for therapeutic targeting of ADAR 54 samples shares therapeutic, targeting
GSE241989 Immunotherapeutic targeting of surfaceome heterogeneity in AML 18 samples shares therapeutic, targeting
GSE250236 Heterogeneous expression of cell surface markers in human myeloma cell lines: implications for disease modelling and therapeutic targeting 18 samples shares therapeutic, targeting
GSE293843 Targeting the ferritinophagy-lysosome axis as a therapeutic vulnerability in gastroenteropancreatic neuroendocrine tumors [RNA-Seq] 10 samples shares therapeutic, targeting
GSE274484 The Long Non-coding RNA Lnc-RAINY Regulates Genes Involved in Radiation Susceptibility Through DNA:DNA:RNA triplex-forming Interactions and has Tumor Therapeutic Potential in Non-small Cell Lung Cancers [ATAC-seq] 16 samples shares therapeutic, non-coding
GSE309595 Longitudinal Analysis of Multiple Myeloma: Therapeutic Response and Immune Microenvironment Dynamics in the Context of Tumor Heterogeneity [scRNA-seq] 270 samples shares therapeutic
GSE215274 Identification of Whole Blood Gene Expression Signature to Predict the Therapeutic Effect of Nintedanib in Idiopathic Pulmonary Fibrosis 177 samples shares therapeutic
GSE275870 Expression profiling of primary and metastatic oral squamous cell carcinoma identifies progression-associated transcriptome changes and therapeutic vulnerabilities 108 samples shares therapeutic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.