GEO series

Targeting p300/CBP abolishes HOXB13 loss-induced lipogenesis and tumor metastasis [ChIP-seq]

GSE296236 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 15 samples Submitted 2025/10/08 Platform GPL20301Platform GPL24676

At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 15 samples

What it is: ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism: Homo sapiens (human)
Samples: 15 samples: 15 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Targeting p300 and CBP abolishes HOXB13-loss-induced lipogenesis and tumor metastasisJCI insight · 2025

Summary (written by the submitters)

HOXB13 is a prostate-specific homeodomain transcription factor that is mainly known as an androgen receptor (AR) cofactor. Recent studies have revealed AR-independent roles of HOXB13 in recruiting HDAC3 to suppress lipogenic programs in prostate cancer (PCa). As such, HOXB13 down-regulation leads to lipid accumulation and tumor metastasis, the molecular mechanisms of which, however, are incompletely understood.

Read the full summary ↓

Here, we demonstrate that p300/CBP co-occupies HOXB13/HDAC3-repressed lipogenic enhancers and is required for their activation and target gene expression upon HOXB13 loss. We found that HOXB13 is down-regulated in metastatic hormone-sensitive PCa compared to their matched primary prostate tumors. HOXB13 loss induces matrix metalloproteinases (MMPs), which mediate HOXB13-depleted cell motility. Critically, CCS1477, a pharmacological inhibitor of p300/CBP, abolished HOXB13-loss-induced lipid accumulation, MMP gene expression, cell motility in vitro, and tumor metastasis in vivo. Taken together, our results suggest HOXB13 as a significant metastasis suppressor and identify p300/CBP as critical therapeutic targets in HOXB13-low metastatic PCa.

Published in

Targeting p300 and CBP abolishes HOXB13-loss-induced lipogenesis and tumor metastasis

Lu X, Peng L, Chu Q et al. · JCI insight 2025 · PMID 41277556 · doi:10.1172/jci.insight.195743

This dataset

Open GSE296236 in finder → Find more human ChIP / ATAC / CUT&Tag datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE296236_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1258253 and SRA study SRP582838. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41277556) ↗

Study design

15 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 15 sample titles: 15 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8967462 LNCaP, HDAC3 ChIP
GSM8967463 LNCaP, HOXB13 ChIP
GSM8967464 LNCaP, p300ChIP
GSM8967465 LNCaP, shctr, DMSO, H3K27ac ChIP
GSM8967466 LNCaP, shctr, CCS1477, H3K27ac ChIP
GSM8967467 LNCaP, shHOXB13, DMSO, H3K27ac ChIP
GSM8967468 LNCaP, shHOXB13, CCS1477, H3K27ac ChIP
GSM8967469 LNCaP, shctr, H3K27ac ChIP
GSM8967470 LNCaP, shp300, H3K27ac ChIP
GSM8967471 LNCaP, shCBP, H3K27ac ChIP
GSM8967472 LNCaP, shp300/CBP, H3K27ac ChIP
GSM8967473 LNCaP, shHOXB13, H3K27ac ChIP
GSM8967474 LNCaP, shHOXB13+shp300, H3K27ac ChIP
GSM8967475 LNCaP, shHOXB13+shCBP, H3K27ac ChIP
GSM8967476 LNCaP, shHOXB13+shp300/CBP, H3K27ac ChIP

Datasets on a similar topic

GSE296250 Targeting p300/CBP abolishes HOXB13 loss-induced lipogenesis and tumor metastasis 43 samples shares hoxb13, p300, cbp, loss-induced, lipogenesis, metastasis, targeting, abolishes
GSE296237 Targeting p300/CBP abolishes HOXB13 loss-induced lipogenesis and tumor metastasis [RNA-seq] 28 samples shares hoxb13, p300, cbp, loss-induced, lipogenesis, metastasis, targeting, abolishes
GSE255132 Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer (ChIP-Seq) 38 samples shares p300, cbp, targeting
GSE255134 Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer 76 samples shares p300, cbp, targeting
GSE237043 Targeting Dependency on a Paralog Pair of CBP/p300 against De-repression of KREMEN2 in SMARCB1-Deficient Cancers 60 samples shares p300, cbp, targeting
GSE255133 Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer (RNA-Seq) 32 samples shares p300, cbp, targeting
GSE291279 Therapeutic Targeting of the p300/CBP Bromodomain Enhances the Efficacy of Immune Checkpoint Blockade Therapy 6 samples shares p300, cbp, targeting
GSE292455 ATF4:p52 Complex Activates Oncogenic Enhancers in Multiple Myeloma via p300/CBP Recruitment to Regulate BACH1 31 samples shares p300, cbp

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.