← BioTransfer GEO Dataset Finder
GEO series

In-vitro cellular tropism and immunomodulatory response to rVSVΔG-ZEBOV-GP in human cells derived from tissues associated with adverse events

GSE296388 Homo sapiens (human) RNA-seq (gene expression) 32 samples Submitted 2026/02/05 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 32 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
32 samples: 6 conditions, mostly with about 6 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
In vitro cellular tropism and immunomodulatory response to rVSVΔG-ZEBOV-GP in human cells derived from tissues associated with adverse eventsMicrobiology spectrum · 2026
Summary (written by the submitters)
The recombinant vesicular stomatitis virus–vectored Zaire Ebola virus glycoprotein (rVSVΔG-ZEBOV-GP) vaccine, while effective and well-tolerated, exhibits notable reactogenicity, manifesting in expected adverse events (AEs) such as fever, headache, and pain, along with rare, unexpected AEs like skin lesions, cutaneous vasculitis, and transient arthritis.
Read the full summary ↓
The presence or absence of AEs following rVSVΔG-ZEBOV-GP vaccination is associated with a specific innate plasma signature. This study aims to elucidate in-vitro the tropism of the vaccine for different cell types derived from tissues previously reported to be involved in the unexpected AEs. Upon in-vitro infection with rVSVΔG-ZEBOV-GP, various cell types such as synoviocytes, fibroblast, keratinocytes and endothelial cells (except chondrocytes) demonstrate productive infection, which in dermal fibroblast triggered the release of many innate plasma signature markers, including keratinocytes’ proinflammatory and proapoptotic cytokines such as OSM and TRAIL. Infected monocytes from buffy coats, activated by infection, produce most innate plasma signature markers. In co-culture, rVSVΔG-ZEBOV-GP-infected monocytes serve as a source to synoviocytes infection, resulting in a distinct kinetics modulation in innate biomarkers (transcription and secretion) and upregulation of specific genes such as NEDD8 and SIGLEC-1, which have been associated with inflammatory arthritis in animal models. Altogether, our work based on in-vitro studies provide insights into the possible mechanisms of rVSVΔG-ZEBOV-GP observed reactogenicity by showing tropism of the vaccine for off target cells derived from AE affected compartments (skin, joints, vessels). Furthermore, in-vitro interaction with infected monocytes modulates the innate response of synoviocytes.
Published in
In vitro cellular tropism and immunomodulatory response to rVSVΔG-ZEBOV-GP in human cells derived from tissues associated with adverse events
Martinez-Murillo P, Alvarez C, Santoro F et al. · Microbiology spectrum 2026 · PMID 41874168 · doi:10.1128/spectrum.00408-25
This dataset
Open GSE296388 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE296388_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1259037 and SRA study SRP583180. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41874168) ↗
Study design
6 conditions, mostly with about 6 replicates each
Monocytes non-infect, ×6 Co-culture infected Monocytes, ×6 Monocytes infect, ×6 Synoviocytes non-infected, ×5 Co-culture non-infected Monocytes, ×5 Synoviocytes infected, ×4

Supports a between-group comparison across 32 samples.

How we read this: 6 replicated groups read from 32 sample titles; they account for 32 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.