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Transcriptomic profile at single cell level of adult lungs expressing exogenous BCL2 driven by three mesenchymal cell-specific drivers

GSE297319 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/06/25 Platform GPL19057
Summary
A transient mesenchymal cell in the lung called alveolar myofibroblast (AMF) plays a key role in alveolar formation during postnatal development. However, AMFs are cleared from the alveoli upon lung maturation. We induce exogenous BCL2 expression in these cells using three lineage-specific drivers (Myh11-CreER, PdgfraCreER, and PdgfrbCreER) to prevent AMF clearance. We use scRNA seq to analyze the transcriptomic changes of AMFs upon BCL2 expression and subsequent persistence in the lung. Additionally, we evaluated the consequences driven by persistent AMFs in other cell lineages at a transcriptional level.
Published in
Apoptosis inhibition reprograms alveolar myofibroblasts toward ductal myofibroblasts
Gacha-Garay MJ, Liu H, Evans SE et al. · bioRxiv : the preprint server for biology 2025 · PMID 40492191 · doi:10.1101/2025.05.26.654588
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Also filed as BioProject PRJNA1263511 and SRA study SRP585630. Searching any of these in the dataset finder brings you back here.

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