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Shifts in embryonic oxygen levels cue heterochrony in limb initiation [scRNA-seq]

GSE298624 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/05/28 Platform GPL34290
Summary
Heterochrony, or the alteration of developmental timing, is an important mechanism of evolutionary change. Avian species display synchronized growth of the forelimbs and hindlimbs, while mammalian species show delayed hindlimb development. We find that mammalian limb heterochrony is evident from the start of limb bud formation, and is associated with heterochronic expression of T-box transcription factors. This heterochronic shift is not due to changes in cis-regulatory sequences controlling T-box gene expression, but unexpectedly, is dependent upon differential oxygen levels to which avian and mammalian embryos are exposed prior to limb initiation, mediated, at least partially, by an NFKB transcription factor, cRel, and hypoxia inducible factor Hif1a. Together, these results provide mechanistic understanding of an important example of developmental heterochrony and exemplify how the maternal environment regulates timing during embryonic development.
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Also filed as BioProject PRJNA1270048 and SRA study SRP588912. Searching any of these in the dataset finder brings you back here.

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