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ATAC-seq of E9.5 Neural Tube defects mouse brain and E9.5 normal mouse brains

GSE299189 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/12/30 Platform GPL24247
Summary
To study the chromatin accessibility in E9.5d mouse brains with neural tube defects or control, we performed ATAC-seq in mouse brains. NTDs mouse model: CD-1 mice (7–8 weeks old) were provided by Beijing Vital River laboratory Animal Technology Co., Ltd, China (no: 110011200105514958, 16-25g). Female CD-1 mice were fed on low folate diet for more than 8 weeks. Sexually matured individuals were mated overnight; vaginal plug was detected at 8:00 am in the following morning and was designated as E0.5 day. NTDs mouse models were induced by intraperitoneal injection with 1.5 mg/kg of MTX (Sigma-Aldrich, USA) on E7.5. On E9.5, pregnant mice were euthanized by cervical dislocation, and the phenotypes of the fetuses were observed under a microscope. All samples with NTDs clinical manifestations were collected for experiments.
Published in
Excessive DNA Double-Strand Breaks-Associated 3D Genome Reorganization Contributes to Neural Tube Defects with Folate Deficiency
Zhang T, Lin L, Li J et al. · Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 · PMID 40966399 · doi:10.1002/advs.202410603
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Also filed as BioProject PRJNA1273049 and SRA study SRP590384. Searching any of these in the dataset finder brings you back here.

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