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Pyruvate, lactate, and hypoxia reprogram murine CD8 T cell chromatin landscape

GSE299227 Mus musculus Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing 24 samples 2026/06/01 GPL17021GPL19057
Summary
To investigate how metabolic and environmental cues shape gene regulation and chromatin accessibility in T cells, CD8 T cells were isolated from mice, activated in vitro, and exposed to lactate, pyruvate, sodium chloride, or hypoxic conditions. On day 3 post-activation, cells were harvested for RNA-seq and ATAC-seq analyses. This integrative approach enabled the identification of transcriptional and epigenetic changes in response to distinct metabolic and oxygen availability conditions
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