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Single cell transcriptomic profiling of eosinophils and airway immune cells in childhood asthma

GSE299751 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/07/24 Platform GPL30173
Summary
To enhance granulocyte recovery in scRNA-seq, we employed nasal lavage samples from children with asthma, leveraging the 10x Genomics Flex platform combined with a customized data processing pipeline. Nasal lavage samples were processed without prior manipulation to avoid technical artifacts such as lysis or stimulation. Granulocyte recovery was optimized using fixation to preserve cell quality and advanced computational techniques to separate low RNA content cells from background noise.
Published in
Single-cell transcriptomic profiling of eosinophils and airway immune cells in childhood asthma
Doni Jayavelu N, Liu AH, Gaberino C et al. · The Journal of allergy and clinical immunology 2025 · PMID 40684957 · doi:10.1016/j.jaci.2025.06.034
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Also filed as BioProject PRJNA1276433 and SRA study SRP591838. Searching any of these in the dataset finder brings you back here.

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