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Age-related gene expression alteration responding to mechanical force and TGFβ signaling [ATAC-seq]

GSE300231 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2026/03/27 Platform GPL34295
Summary
The aging process significantly alters the biomechanical properties of fibroblasts, impacting their cytoskeletal and chromatin organization. These changes are pivotal in modulating cellular responses to mechanical and biochemical stimuli. This study investigates the hypothesis that aging leads to fibroblasts’ divergent adaptation mechanisms to external stimuli. Herein, fibroblasts from young and old cohorts were embedded in 3D collagen gels and subjected to tension and Transforming Growth Factor beta (TGFb) stimulation. The study revealed that mechanical forces and aging exert profound effects on chromatin architecture. Young cells adapted more rapidly to mechanical stress and showed a heightened responsiveness to TGFβ compared to older cells. The identification of key transcription factors implicated in age-dependent responses was further examined, assessing their potential in cellular rejuvenation.
Published in
Chromatin accessibility regulates age-dependent nuclear mechanotransduction
Liao Y, Yuan L, Sornapudi TR et al. · Proceedings of the National Academy of Sciences of the United States of America 2026 · PMID 41886372 · doi:10.1073/pnas.2522217123
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Also filed as BioProject PRJNA1279933 and SRA study SRP593388. Searching any of these in the dataset finder brings you back here.

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