← BioTransfer GEO Dataset Finder
GEO series

HERVHLin modulates pluripotency and protects the genome from active retroelements by rewiring the conserved LIN28A/let-7 pathway

GSE300465 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2025/07/01 Platform GPL34284
Summary
Sequential waves of coding transcript expression mark genome reactivation during embryonic development and reprogramming to pluripotency. We additionally observed two distinct waves of non-coding retrotransposable element (RE) expression: an initial wave of younger, partially active REs (LINE-1, SVA, and Alu), followed by older HERVH elements. These waves are not merely correlative, as HERVH depletion leads to an increase in transposition of L1, Alu, and SVA elements. We identified a great apes-specific subgroup of HERVH (N=101), termed HERVHLin, characterized by a motif with duplicated LIN28 binding sites, which alone inhibits L1 transposition. HERVHLin transcripts bind to LIN28 and allow let-7 microRNA to mature, rewiring the conserved LIN28-let-7 regulatory axis. Full locus HERVHLin knockout (KO) results in increased LINE-1 transposition, rescued by the let-7 mimic. HERVHLin KO presents an EMT-like phenotype, downregulation of RE-defense and pluripotency-specific genes, underscoring the role of HERVHLin in genome protection of pluripotent stem cells.
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE300465_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1281005 and SRA study SRP594062. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 9 more — browse all 9 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.