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Long non-coding RNA AFAP1-AS1 promotes alternative splicing of AXIN2 by facilitating SRSFs phase separation to induce drug resistance in lung adenocarcinoma

GSE300659 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/12/04 Platform GPL24676
Summary
In this study, we identified that the long non-coding RNA actin filament associated protein 1 antisense RNA 1 (AFAP1-AS1), which is highly expressed in lung adenocarcinoma, regulates RNA alternative splicing by recruiting splicing factors such as serine and arginine rich splicing factor 1 and 3 (SRSF1 and SRSF3), promoting their liquid-liquid phase separation. To investigate the effect of AFAP1-AS1 on alternative splicing, we overexpressed and knocked down AFAP1-AS1 in lung adenocarcinoma cell lines and obtained all regulated alternative splicing events through RNA seq, exploring the potential mechanism of AFAP1-AS1 promoting the occurrence and development of lung adenocarcinoma.
Published in
Long non-coding RNA AFAP1-AS1 promotes alternative splicing of AXIN2 by facilitating SRSFs phase separation to induce drug resistance in lung adenocarcinoma
Ouyang J, Zhang Y, Hou X et al. · Molecular cancer 2025 · PMID 41261425 · doi:10.1186/s12943-025-02425-4
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Also filed as BioProject PRJNA1281618 and SRA study SRP594281. Searching any of these in the dataset finder brings you back here.

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