GEO series
Comparative analysis of replicative and doxorubicin-induced senescence identifies conserved transcriptomic signatures in aging fibroblasts [RNA-Seq1]
GSE301164
Homo sapiens
Expression profiling by high throughput sequencing
21 samples
2025/12/02
GPL29480
Summary
We established parallel replicative and doxorubicin-induced senescence models in human BJ fibroblasts, demonstrating progressive morphological changes and SA-β-gal activity increases across passages (P3-P33). Molecular analyses confirmed expected senescence markers: p21/p53 upregulation and LMNB1 downregulation at RNA/protein levels. Transcriptomic profiling identified 734 upregulated (extracellular matrix organization) and 195 downregulated (cell cycle) genes. Comparative lncRNA analysis revealed 23 consistently upregulated candidates, with PURPL showing the most dramatic senescence-associated induction. These validated models provide a robust platform for mechanistic aging studies, with PURPL emerging as a key regulator of cellular senescence.
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Paper (PMID 41107828) ↗
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