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Human cerebral organoids model tumor initiation and infiltration in an autologous astrocyte-supported setting [RNA-Seq]

GSE301276 Homo sapiens Expression profiling by high throughput sequencing 5 samples Submitted 2025/07/01 Platform GPL24676
Summary
Efforts to efficiently target brain tumors are constrained by the dearth of appropriate models to study tumor behavior toward treatment approaches as well as potential side effects to the surrounding normal tissue. We established a reproducible cerebral organoid model of brain tumorigenesis in an autologous setting by overexpressing c-MYC, a common oncogene in brain tumors. GFP+/c-MYChigh cells were isolated from tumor organoids and used in two different approaches: GFP+/c-MYChigh cells co-cultured with cerebral organoid slices or fused as spheres to whole organoids. GFP+/c-MYChigh cells used in both approaches exhibited tumor-like properties, including an immature phenotype and a highly proliferative and invasive potential. We demonstrate that the latter is influenced by astrocytes supporting the GFP+/c-MYChigh cells while X-ray irradiation significantly kills and impairs tissue infiltration of GFP+/c-MYChigh cells. In summary, the model represents major features of tumorous and adjacent normal tissue and may be used to evaluate appropriate cancer treatments.
Published in
Human cerebral organoids model tumor initiation and infiltration in an autologous astrocyte-supported setting
Schickel E, Bender T, Kaysan L et al. · iScience 2025 · PMID 40917877 · doi:10.1016/j.isci.2025.113334
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Also filed as BioProject PRJNA1284666 and SRA study SRP596343. Searching any of these in the dataset finder brings you back here.

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