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Epigenetic Reactivation of CNS Endothelial Developmental Programs Triggers Adult Brain Angiogenesis, Promotes Post-Stroke Revascularization and Neuroregeneration [RNAseq-Stroke]

GSE301448 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2025/07/06 Platform GPL34290
At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
18 samples: 6 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Epigenetic Reactivation of CNS Endothelial Developmental Programs Triggers Adult Brain Angiogenesis, Promotes Post-Stroke Revascularization and Neuronal RegenerationbioRxiv : the preprint server for biology · 2025
Summary (written by the submitters)
We identified the role of brain endothelial cell Hdac2 and Ezh2 mediated epigenetic mechanism in maintaining blood brain barrier in adult mice. Also, we we studied the impact of reactivation of brain endothelial cell Wnt/β-catenin signaling in adult mice. .We evaluated derepression of Hdac2 mediated epigenetic mechanism in imice model of schemic stroke.
Published in
Epigenetic Reactivation of CNS Endothelial Developmental Programs Triggers Adult Brain Angiogenesis, Promotes Post-Stroke Revascularization and Neuronal Regeneration
Thomas S, Chandran C, Ahirwar LK et al. · bioRxiv : the preprint server for biology 2025 · PMID 40894561 · doi:10.1101/2025.08.15.670554
This dataset
Open GSE301448 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE301448_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1285181 and SRA study SRP597074. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40894561) ↗
Study design
6 conditions, mostly in triplicate
Brain Cortical Endothelial cells from Sham mi… ×3 Brain Cortical Endothelial cells from Sham mi… ×3 Brain Cortical Endothelial cells from MCAO mi… ×3 Brain Cortical Endothelial cells from MCAO mi… ×3 Brain Cortical Endothelial cells from MCAO-Hd… ×3 Brain Cortical Endothelial cells from MCAO-Ez… ×2 Brain Cortical Endothelial cells from MCAO-Ez… ×1

Supports a between-group comparison across 17 samples.

How we read this: 6 replicated groups read from 18 sample titles; they account for 17 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.