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Unique sequence features define epigenetic longevity of inflammatory memory [scATAC-seq]

GSE301537 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/12/01 Platform GPL24247
Summary
Many tissues harbor epigenetic memories of inflammation, which heighten sensitivity to diverse future assaults. Whether and how these adaptations are sustained through time and cell division remain poorly understood. Here, we show that epidermal stem cells store functional epigenetic records of a psoriatic skin flare that last a lifetime. Applying deep learning to interrogate these chromatin dynamics, we unearth CpG dinucleotide density as a major driver of memory persistence. Molecularly, while unnecessary for stress-induced chromatin opening, CpG dinucleotides thereafter become essential, reinforcing accessibility across cellular generations through a signature integrating DNA demethylation, methylation-sensitive transcription factors, sequence-intrinsic nucleosome disaffinity and nucleosome-destabilizing histone variant H2A.Z. Thus, inherent CpG enrichment within DNA sequence confers enduring adaptability to environmentally sensitive cis-regulatory elements, with profound consequences to long-term tissue fitness.
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Direct links to NCBI, no account and no request form: the whole study as GSE301537_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1285399 and SRA study SRP597878. Searching any of these in the dataset finder brings you back here.

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