Research on the dynamic changes of signaling pathways during SARS-CoV-2 infection of host cells

SARS-CoV-2 continues to pose a substantial public health challenge owing to its high transmissibility and rapid mutation capacity. The immune regulation of SARS-CoV-2 is a complex process involving both viral immune evasion mechanisms and the dynamic balance of host immune responses. We performed RNA-seq sequencing on host cells at different time points post-infection, followed by pairwise comparative enrichment analysis and clustering analysis, ultimately identifying the regulatory changes in signaling pathways during the infection process.