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Stromal Transcriptomics Uncover LIF as a Key Effector in High Tumor Budding Triple-Negative Breast Cancer

GSE302177 Homo sapiens Expression profiling by high throughput sequencing 13 samples 2025/09/10 GPL16791
Summary
The overexpressed genes in FAP-positive CAFs from high TB-TNBCs included ZNF235, ANKRD30B, SLC26A2, SPDYC, CDKN1C, LIF, and FAM83A. The secreted LIF was found in 41.1% of TNBC cases with high levels detected in CAFs, while 26.2% of cases showed high LIF production in cancer cells. The recombinant LIF significantly promoted breast cancer cell migration and enhanced PD-L1 expression, effects that were attenuated by EC359. This study highlights the correlation of high TB in TNBC with poor prognosis and upregulation of key signaling pathways in CAFs. FAP-positive CAFs overexpress genes like LIF, which promotes cell migration and PD-L1 expression. LIF inhibition reduces these effects, suggesting LIF as a potential therapeutic target in TB-associated TNBC progression and immunotherapy.
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NCBI GEO page ↗ Paper (PMID 41290930) ↗ {# Names what the click gives you. "Open in finder" meant nothing to a visitor who arrived from a search engine and has never seen the tool. #} Find more human RNA-seq datasets →
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