← BioTransfer GEO Dataset Finder
GEO series

CDK8 inhibition sequesters Med1 at EWS-FLI enhancers [ATAC-seq]

GSE302424 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2026/07/09 Platform GPL30173
Summary
Ewing sarcoma is characterized by a chromosomal translocation resulting in the fusion protein EWSR1-FLI1. To identify small molecules that selectively impair the oncogenic transcriptional program enacted by EWS-FLI1 we employed a novel phenotypic screen. Our screening hits were enriched for inhibitors of cyclin dependent kinase 8/19. Inhibition of CDK8/19 resulted in a proliferation defect in Ewing sarcoma cells and a reduction in growth of Ewing sarcoma xenografts. We performed a genome wide pooled CRISPR screen that suggested that small molecule inhibition of CDK8/19 acted in a gain-of-function manner that did not replicate the effects of CDK8/19 genetic knockout. Through biochemical studies and chromatin profiling, we discovered that CDK8/19 inhibitors act through a novel trapping mechanism in which the inhibitor prevents dissociation of the CDK8 kinase from core Mediator. This mechanism has broad implications for the development of small molecules to target transcriptional regulation.
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE302424_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1290401 and SRA study SRP600261. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all human ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.