GEO series

Functional Comparison to Ezh2 Reveals PRC2-Independent Functions of Jarid2 in Hematopoietic Stem Cell Lineage Commitment

GSE302605 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 23 samples Submitted 2025/11/17 Platform GPL34290

At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 23 samples

What it is: ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism: Mus musculus (mouse)
Samples: 23 samples: 23 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Comparison with Ezh2 reveals the PRC2-dependent functions of Jarid2 in hematopoietic stem Cell lineage commitmentStem cell reports · 2026

Summary (written by the submitters)

Our previous studies showed the Polycomb Repressive Complex 2 (PRC2) co-factor Jarid2 represses self-renewal transcriptional networks in mouse multipotent progenitor cells (MPPs). But only a fraction of de-repressed HSC-specific genes were associated with loss of H3K27me3, implying Jarid2 may have non-canonical (PRC2-indpendent) in hematopoiesis.

Read the full summary ↓

Here we sought to delineate any PRC2-independnent functions by comparing stem and progenitor cells genetically deficient for either Jarid2 or Ezh2. Loss of Ezh2 leads to increased myeloid differentiation in transplantation assays. In contrast, loss of Jarid2 enhanced T-cell output. Single cell transcriptomics showed while loss of Jarid2 had minimal impact across progenitor populations, loss of Ezh2 led to accumulation of lymphoid-biased MPP4 cells and B-cell progenitors in the bone marrow. Functional assays confirmed a differentiation block at the pre-pro B-cell stage. The maturational arrest of Ezh2-deficient B-cell progenitors contrasts with increased T-cell output from loss of Jarid2, suggesting Jarid2 has non-canonical functions in hematopoiesis.

Published in

Comparison with Ezh2 reveals the PRC2-dependent functions of Jarid2 in hematopoietic stem Cell lineage commitment

Bjeije H, Han W, Lin S et al. · Stem cell reports 2026 · PMID 41650958 · doi:10.1016/j.stemcr.2026.102793

This dataset

Open GSE302605 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE302605_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1291502 and SRA study SRP600896. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41650958) ↗

Study design

23 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 23 sample titles: 23 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9107458 Single_Cell_RNA_seq_500K_WBM_1TX-Control_KSL_Cells
GSM9107459 Single_Cell_RNA_seq_500K_WBM_1TX-Ezh2_KO_1_KSL_Cells
GSM9107460 Single_Cell_RNA_seq_500K_WBM_1TX-Ezh2_KO_2_KSL_Cells
GSM9107461 Single_Cell_RNA_seq_500K_WBM_1TX-Jarid2_KO_1_KSL_Cells
GSM9107462 Single_Cell_RNA_seq_500K_WBM_1TX-Jarid2_KO_2_KSL_Cells
GSM9107463 Single_Cell_multi-omics_seq_GEX_baseline-Control_Male_KSL_Cells
GSM9107464 Single_Cell_multi-omics_seq_ADT_baseline-Control_Male_KSL_Cells
GSM9107465 Single_Cell_multi-omics_seq_GEX_baseline-Control_Female_KSL_Cells
GSM9107466 Single_Cell_multi-omics_seq_ADT_baseline-Control_Female_KSL_Cells
GSM9107467 Single_Cell_multi-omics_seq_GEX_baseline-Control_Jarid2_KO_Male_KSL_Cells
GSM9107468 Single_Cell_multi-omics_seq_ADT_baseline-Control_Jarid2_KO_Male_KSL_Cells
GSM9107469 Single_Cell_multi-omics_seq_GEX_baseline-Control_Jarid2_KO_Female_KSL_Cells
GSM9107470 Single_Cell_multi-omics_seq_ADT_baseline-Control_Jarid2_KO_Female_KSL_Cells
GSM9107471 Single_Cell_multi-omics_seq_GEX_baseline-Control_Ezh2_KO_Male_KSL_Cells
GSM9107472 Single_Cell_multi-omics_seq_ADT_baseline-Control_Ezh2_KO_Male_KSL_Cells
GSM9107473 Single_Cell_multi-omics_seq_GEX_baseline-Control_Ezh2_KO_Female_KSL_Cells
GSM9107474 Single_Cell_multi-omics_seq_ADT_baseline-Control_Ezh2_KO_Female_KSL_Cells
GSM9292645 H3K27me3_3 million_WBM_2TX_Control_1_KSL_Cells
GSM9292646 H3K27me3_3 million_WBM_2TX_Control_2_KSL_Cells
GSM9292647 H3K27me3_3 million_WBM_2TX_Jarid2_KO_1_KSL_Cells
GSM9292648 H3K27me3_3 million_WBM_2TX_Jarid2_KO_2_KSL_Cells
GSM9292649 H3K27me3_3 million_WBM_2TX_Ezh2_KO_1_KSL_Cells
GSM9292650 H3K27me3_3 million_WBM_2TX_Ezh2_KO_2_KSL_Cells

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GSE278208 STING Mediates Increased Self-Renewal and Lineage Skewing in DNMT3A-Mutated Hematopoietic Stem/Progenitor Cells 40 samples shares hematopoietic, lineage
GSE293107 SKIDA1 transiently sustains MLL::ENL-Expressing hematopoietic progenitors during neonatal stages and promotes B-lineage priming [RNA-Seq] 24 samples shares hematopoietic, lineage
GSE310548 Distinct contributions of Etv2+ and Flk1+ progenitors to endothelial, hematopoietic, and cardiac lineages [RNA-seq] 21 samples shares hematopoietic, lineage
GSE288012 Genome-wide screen identifies Runx2 as a novel regulator of hematopoietic stem cell expansion and T-cell commitment 20 samples shares hematopoietic, commitment
GSE222941 Endomucin marks quiescent long-term multi-lineage repopulating hematopoietic stem cells and is essential for their transendothelial migration [RNA-seq: wildtype_EMCN] 11 samples shares hematopoietic, lineage
GSE214191 Oxidative Stress Converts Benign Lesions to Cancer via a Self-Amplifying NRF2-EZH2 Loop 63 samples shares ezh2

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