GEO series

A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types (DroNc-Seq)

GSE303082 Mus musculus (mouse) Single-cell RNA-seq 14 samples Submitted 2025/12/16 Platform GPL19057

At a glance

Single-cell RNA-seq · mouse · 14 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism: Mus musculus (mouse)
Samples: 14 samples: 14 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell typesResearch square · 2025

Summary (written by the submitters)

The paraventricular hypothalamus (PVH) controls many behavioral and physiologic processes, including appetite, social behavior, autonomic outflow, and pituitary hormone secretion. However, molecular markers for functionally-specific PVH neuron projections remain largely undefined, and a complete census of PVH cell types has not been established.

Read the full summary ↓

Therefore, we performed extensive single-cell/nucleus RNA sequencing to identify unique neuronal subtypes and multiplexed error-robust fluorescence in situ hybridization (MERFISH) to validate and map them spatially. Our spatial transcriptomic atlas resolved 26 Sim1+ and 29 GABAergic neuron populations from the PVH and surrounding areas, revealing multiple subtypes not described previously and distinct transcriptional programs between neuroendocrine and centrally-projecting neurons. Additionally, projection-based profiling determined neuronal subtypes that project to the parabrachial region (PB) and spinal cord, helping to identify PVH populations that regulate satiety and sympathetic nervous system activity, respectively. Notably, PB-projecting PVH neurons expressing bombesin-like receptor 3 (Brs3) induce satiety, and their silencing causes obesity. Together, this atlas fills a critical gap by contributing high-resolution PVH spatial and circuit-based gene expression profiles, representing a valuable resource for the field of homeostasis.

Published in

A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types

Resch J, Li Y, Butler T et al. · Research square 2025 · PMID 41282229 · doi:10.21203/rs.3.rs-7895391/v1

This dataset

Open GSE303082 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE303082_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1293954 and SRA study SRP602315. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41282229) ↗

Study design

14 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 14 sample titles: 14 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9117436 PVH nuclei, male, fasted, sample 8, drop 1, RT-rxn A, lib. 1
GSM9117437 PVH nuclei, male, fasted, sample 8, drop 1, RT-rxn B, lib. 1
GSM9117438 PVH nuclei, male, fasted, sample 8, drop 2, RT-rxn A, lib. 1
GSM9117439 PVH nuclei, male, fasted, sample 8, drop 2, RT-rxn B, lib. 1
GSM9117440 PVH nuclei, male, fasted, sample 8, drop 3, RT-rxn A, lib. 1
GSM9117441 PVH nuclei, male, fasted, sample 8, drop 3, RT-rxn B, lib. 1
GSM9117442 PVH nuclei, male, fed, sample 8, drop 1, RT-rxn A, lib. 1
GSM9117443 PVH nuclei, male, fed, sample 8, drop 2, RT-rxn A, lib. 1
GSM9117444 PVH nuclei, male, fed, sample 8, drop 2, RT-rxn B, lib. 1
GSM9117445 PVH nuclei, male, fed, sample 8, drop 3, RT-rxn A, lib. 1
GSM9117446 PVH nuclei, male, fed, sample 8, drop 3, RT-rxn B, lib. 1
GSM9117447 PVH nuclei, female, sample 9, drop 1, RT-rxn A&B, lib. 1
GSM9117448 PVH nuclei, female, sample 9, drop 2, RT-rxn A&B, lib. 1
GSM9117449 PVH nuclei, female, sample 9, drop 3, RT-rxn A&B, lib. 1

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