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Spatiotemporal single-cell atlas of suture stem cell dynamics in craniosynostosis [scRNA-Seq]

GSE303460 Mus musculus (mouse) Single-cell RNA-seq 14 samples Submitted 2026/05/20 Platform GPL24247
At a glance

Single-cell RNA-seq · mouse · 14 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism
Mus musculus (mouse)
Samples
14 samples: 6 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Spatiotemporal single-cell atlas of suture stem cell dynamics in craniosynostosisStem cell research & therapy · 2026
Summary (written by the submitters)
Craniosynostosis is a congenital craniofacial disorder marked by premature suture fusion and aberrant skull morphogenesis. Yet the cellular dynamics and regulatory mechanisms of suture mesenchymal stem cells (SuSCs) in this disease remain largely unknown. Here, we present a comprehensive spatiotemporal atlas of SuSCs integrating single-cell and 2 μm-resolution spatial transcriptomics in Fgfr2C342Y/+, a model that recapitulates features of human Crouzon syndrome, alongside wild-type controls across three key developmental stages (E14.5, E18.5, and P3).
Read the full summary ↓
By integrating morphology-based cell segmentation with machine-learning classification, we achieved single-cell spatial resolution in mapping Visium HD data. Leveraging this resource, we reveal disruptions in SuSC fate, niche composition, and intercellular signaling across developmental stages in craniosynostotic sutures. Downstream analyses identify Foxa3 as a master transcriptional regulator of these fate shifts and implicate specific immune- and suture meningeal fibroblast–derived signals in driving pathological ossification. Together, our work fills a gap in disease-model single-cell spatial-omics, providing a high-resolution roadmap of SuSC dynamics in craniosynostosis and uncovering candidate molecular targets for future therapeutic strategies.
Published in
Spatiotemporal single-cell atlas of suture stem cell dynamics in craniosynostosis
Chen X, Lai C, He T et al. · Stem cell research & therapy 2026 · PMID 42071254 · doi:10.1186/s13287-026-04987-6
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE303460_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1295019 and SRA study SRP602834. Searching any of these in the dataset finder brings you back here.

Study design
6 conditions, mostly in duplicate
P3-CS ×3 P3-WT ×3 E14.5-CS ×2 E14.5-WT ×2 E18.5-CS ×2 E18.5-WT ×2

Supports a between-group comparison across 14 samples.

How we read this: 6 replicated groups read from 14 sample titles; they account for 14 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.