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Human gastric extracellular matrix hydrogels maintain organoid growth and reduce gene expression associated with epithelial-mesenchymal transition and inflammation

GSE303526 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2026/07/26 Platform GPL30173
Summary
The extracellular matrix (ECM) material that organoids are maintained in provides crucial signals to the epithelial cells, regulating their function and growth. We here describe the application of human gastric ECM (hgECM) for human gastric organoid culture. HgECM was prepared from human gastric tissue by decellularization and pepsin digestion. Transcriptional profiles of HGOs grown in hgECM and Matrigel were compared using single cell RNA sequencing (scRNAseq). scRNAseq revealed that cell type composition of HGOs in each ECM was comparable. However, HGOs in hgECM had reduced expression of genes associated with stem cells, epithelial-mesenchymal transition, stress, and inflammation compared to HGOs grown in Matrigel. Conversely, HGO culture in hgECM increased expression of gastric tissue-specific genes and of genes associated with growth and metabolism.
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Also filed as BioProject PRJNA1295566 and SRA study SRP603093. Searching any of these in the dataset finder brings you back here.

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