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Stromal Cells from Endometriosis Patients Reflect Lesion Type-Specific Heterogeneity

GSE303635 Homo sapiens (human) RNA-seq (gene expression) 20 samples Submitted 2025/12/17 Platform GPL34284
At a glance

RNA-seq (gene expression) · human · 20 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
20 samples: 5 × C vs 5 × DE vs 5 × OMA vs 5 × SFGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Endometrial Stromal Cells from Endometriosis Patients Reflect Lesion-Type-Specific HeterogeneityCells · 2025
Summary (written by the submitters)
Endometriosis shows significant lesion heterogeneity, but pathophysiological mechanisms leading to differences in clinical presentation are poorly understood. Here we utilized primary stromal cells from different types of endometriotic lesions to better understand the molecular mechanisms that lead to endometriosis heterogeneity. For this project, endometrial stromal cells (ESCs) were obtained from eutopic endometrium (EU), endometrioma (OMA), superficial (SF) and deep (DE) endometriosis lesions and analyzed in vitro for their behavior regarding proliferation, migration, and contractility.
Read the full summary ↓
RNA sequencing was performed to explore the molecular mechanism underlying the observed changes.
Published in
Endometrial Stromal Cells from Endometriosis Patients Reflect Lesion-Type-Specific Heterogeneity
Rodriguez Gutierrez D, Spalinger MR, Astourian A et al. · Cells 2025 · PMID 41369380 · doi:10.3390/cells14231891
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE303635_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1295965 and SRA study SRP603316. Searching any of these in the dataset finder brings you back here.

Study design
5 × C vs 5 × DE vs 5 × OMA vs 5 × SF

Supports a between-group comparison across 20 samples.

How we read this: 4 replicated groups read from 20 sample titles; they account for 20 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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