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Inactivation of phosphatidylinositol 4-kinase IIIalpha (PI4KA) by GSK-A1 inhibitor in HEK293 cells

GSE303723 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/12/04 Platform GPL16791
Summary
Phosphatidylinositol 4-kinases (PI4Ks) are lipid kinase enzymes that play important roles in cellular signaling, membrane trafficking, viral replication and lipid metabolism. They catalyze the phosphorylation of phosphatidylinositol (PI) to produce phosphatidylinositol 4-phosphate (PI4P) at various organelle membranes. Among the four isoforms of PI4Ks, PI4KA is localized at the plasma membrane where it generates the lipid, PI4P. GSK-A1 is a well-characterized and specific inhibitor of PI4KA. In this study, we investigated transcriptional changes that occur following prolonged (24 h) treatment with GSK-A1. This RNA-seq analysis revealed significant changes in the mRNA levels of a distinct set of proteins, including transcription factors and small GTP-binding proteins. These changes were analyzed in the context of overall lipid level alterations induced by GSK-A1 treatment, as determined by lipidomic analyses.
Published in
Phosphatidylserine and RhoB connect phosphatidylinositol 4-phosphate and phosphatidic acid metabolism at the plasma membrane
Huang S, Kim YJ, Cao X et al. · bioRxiv : the preprint server for biology 2025 · PMID 41256676 · doi:10.1101/2025.09.30.679611
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Direct links to NCBI, no account and no request form: the whole study as GSE303723_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1297159 and SRA study SRP603796. Searching any of these in the dataset finder brings you back here.

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