RNA-seq and MeRIP-seq comparison of Mettl3 knockout basal cell derived organoids

Airway basal stem cells are critical for maintaining epithelial homeostasis and repairing tissue damage, yet the epitranscriptomic mechanisms governing their stemness remain poorly understood. We show that METTL3 deficiency in murine airway basal cells leads to aberrant differentiation, loss of stemness, and impaired organoid formation. RNAseq and MeRIP-seq reveal that METTL3 directly targets the mRNA of Ninj1 for m6A modification, promoting its degradation. Loss of METTL3 leads to Ninj1 upregulation.