← BioTransfer GEO Dataset Finder
GEO series

Neuronal Necroptosis Drives Neuroinflammation and Cognitive Decline Independent of Neuronal Cell Death

GSE304069 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2025/07/30 Platform GPL19057
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 12 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Neuronal Necroptosis Drives Neuroinflammation and Cognitive Decline Independent of Neuronal Cell DeathAging and disease · 2025
Summary (written by the submitters)
This study investigates transcriptomic changes in a novel neuron-specific Mlkl knockin (nMlkl-KI) mouse model following tamoxifen-induced MLKL expression in neurons. Mlkl-KI mice were generated on a C57BL/6J background using a transgene construct containing 3xFLAG-tagged Mlkl cDNA inserted into the Rosa26 locus with a loxP-flanked stop cassette.
Read the full summary ↓
Hemizygous Mlkl-KI females were crossed with homozygous Slick-H-Cre (Thy1-CreERT2-EYFP) males to produce mice expressing MLKL selectively in neurons upon tamoxifen induction. At 45 days of age, mice were treated with tamoxifen (60 mg/kg, intraperitoneally) or corn oil for 5 consecutive days. Brain cortex tissue was collected and RNA was extracted for sequencing. At 12-months of age, nMlkl-KI mice exhibited significant cognitive impairment compared to control mice as measured by a continuous homecage discrimination learning with the Noldus PhenoTyper. Unbiased stereological analysis revealed no loss in neuronal number in the cortex and hippocampus, suggesting neuronal dysfunction rather than neuronal death was responsible for the reduced cognition observed in the nMlkl-KI mice. Transcriptomic analysis of the cortex revealed an upregulation of pathways associated with age-related neurodegenerative diseases as well as chemokine and TNF signaling in the nMlkl-KI mice.
Published in
Neuronal Necroptosis Drives Neuroinflammation and Cognitive Decline Independent of Neuronal Cell Death
Thadathil N, Wolf NA, Wolf R et al. · Aging and disease 2025 · PMID 41082292 · doi:10.14336/AD.2025.0805
This dataset
Open GSE304069 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE304069_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1298866 and SRA study SRP604736. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41082292) ↗
Study design
12 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 12 sample titles: 12 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.