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Targeting the AKT–EphA2 Axis and Cell–Cell Adhesion Enhances Anoikis Sensitivity in Cancer Cells

GSE304274 Homo sapiens (human) RNA-seq (gene expression) 28 samples Submitted 2025/08/11 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 28 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
28 samples: 13 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Targeting the Akt-EphA2 axis and cell-cell adhesion enhances anoikis sensitivity in cancer cellsScientific reports · 2026
Summary (written by the submitters)
Rigidity sensing enables cells to respond to extracellular matrix stiffness and governs survival and apoptotic decisions. Tropomyosin 2.1 (Tpm2.1), a key actin-binding protein, is frequently downregulated in cancer. While re-expressing Tpm2.1 restores rigidity sensing and limits anchorage-independent growth, its ability to fully trigger anoikis, apoptosis induced by loss of matrix attachment, remains unclear.
Read the full summary ↓
Here, we show that although Tpm2.1 overexpression reduces AKT activation and suppresses survival signaling in suspended breast cancer cells, most cells remain viable without matrix attachment. Time-resolved transcriptomic profiling of apoptotic and non-apoptotic suspended cells revealed compensatory survival programs, including reactivation of PI3K–Akt signaling and upregulation of intercellular adhesion molecules such as ICAM1. Importantly, anoikis resistance can be overcome. Pharmacological inhibition of AKT, EphA2, or ICAM1 in Tpm2.1-expressing cells significantly increases apoptosis under suspension conditions. These findings highlight that while rigidity sensing restoration alone may be insufficient to induce anoikis, it sensitizes cancer cells to targeted therapies, revealing actionable vulnerabilities to eliminate detached tumor cells.
Published in
Targeting the Akt-EphA2 axis and cell-cell adhesion enhances anoikis sensitivity in cancer cells
Vivante AG, Dwivedi N, Sheetz MP et al. · Scientific reports 2026 · PMID 41582276 · doi:10.1038/s41598-026-37327-y
This dataset
Open GSE304274 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE304274_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1299805 and SRA study SRP605879. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41582276) ↗
Study design
13 conditions, mostly in duplicate
MDA-MB-231 cells, control, adherent, biol ×2 MDA-MB-231 cells with Tpm2.1 overexpression, … ×2 MDA-MB-231 cells, control, 5 hours in suspens… ×2 MDA-MB-231 cells, control, 1 day in suspensio… ×2 MDA-MB-231 cells, control, 2 days in suspensi… ×2 MDA-MB-231 cells with Tpm2.1 overexpression, … ×2 MDA-MB-231 cells with Tpm2.1 overexpression, … ×2 MDA-MB-231 cells with Tpm2.1 overexpression, … ×2 +7 more

Supports a case/control comparison: 18 samples read as cases, 10 as controls.

How we read this: 13 replicated groups read from 28 sample titles; they account for 26 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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