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Transcriptomic analysis of KMT5A-knockout healthy volunteer peripheral blood CD8+ T cells under activated conditions

GSE304753 Homo sapiens Expression profiling by high throughput sequencing 5 samples Submitted 2025/12/02 Platform GPL24676
Summary
This study aims to investigate the role of Lysine Methyltransferase 5A (KMT5A, also known as SETD8) in CD8+ T cell biology. CD8+ T cells were isolated from healthy volunteer PBMCs using magnetic bead-based negative selection. Isolated cells were subjected to CRISPR-Cas9 mediated gene editing targeting KMT5A (KMT5A-KO group) or a non-targeting control guide RNA (control group, CTRL) via ribonucleoprotein (RNP) complex electroporation. Cells were harvested at 72 hours post-electroporation for RNA extraction. RNA sequencing (RNA-seq) libraries were constructed using the Illumina TruSeq Stranded mRNA kit and sequenced on an Illumina platform to generate paired-end reads. Transcriptome profiles of KMT5A-KO CD8+ T cells were compared to those of CTRL cells to identify differentially expressed genes (DEGs) and elucidate potential pathways regulated by KMT5A in this cell type.
Published in
Genome editing or small molecule inhibition of KMT5A in CAR-T cells enhances antitumor immunity
Tian X, Liu G, Wang Q et al. · Journal for immunotherapy of cancer 2025 · PMID 40940133 · doi:10.1136/jitc-2025-012160
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Direct links to NCBI, no account and no request form: the whole study as GSE304753_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 5 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1302454 and SRA study SRP607043. Searching any of these in the dataset finder brings you back here.

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