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Transcriptomic profiling of DHA-treated human chondrocytes

GSE304835 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/10/31 Platform GPL24676
Summary
This study aimed to investigate the regulatory effects of docosahexaenoic acid (DHA) on lipid metabolism in human osteoarthritic chondrocytes. All cells were pretreated with 10 ng/mL interleukin-1β (IL-1β) for 24 hours to simulate the inflammatory environment of osteoarthritis. Subsequently, cells were divided into two groups: one treated with 25 μM DHA for 48 hours and the other with PBS as control. Total RNA was extracted and subjected to strand-specific paired-end RNA sequencing to examine transcriptomic changes related to lipid metabolic pathways.
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Direct links to NCBI, no account and no request form: the whole study as GSE304835_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1303019 and SRA study SRP607315. Searching any of these in the dataset finder brings you back here.

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