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Single-nucleus multiome ATAC-seq and RNA-seq analysis of GCM1 knockout in rotational human tropholast organoids

GSE305070 Homo sapiens Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2026/03/09 Platform GPL28038
Summary
Single nuclei were isolated from organoids to investigate the effects of GCM1 knockout and AAVS1 knockout (as control) on chromatin accessibility and gene expression profiles. Approximately 10,000 nuclei per sample were processed using 10x Genomics Chromium Next GEM Single Cell Multiome ATAC + Gene Expression kit, enabling simultaneous profiling of chromatin accessibility and gene expression from individual nuclei. Raw sequencing data were processed using Cell Ranger ARC to generate filtered feature-barcode matrices. Data analysis was performed in R (v4.3+) using Seurat (v4) and Signac packages.
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Direct links to NCBI, no account and no request form: the whole study as GSE305070_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1304391 and SRA study SRP608205. Searching any of these in the dataset finder brings you back here.

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