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Identification of Detachment-Responsive Transcripts in Ovarian Cancer

GSE305375 Homo sapiens (human) RNA-seq (gene expression) 36 samples Submitted 2026/01/31 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 36 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Topic
Ovarian cancerThis series is part of our curated briefing on it.
Samples
36 samples: 9 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
RHOV Is a Detachment-Responsive Rho GTPase Necessary for Ovarian Cancer Peritoneal MetastasisCancer research · 2026
Summary (written by the submitters)
Transcoelomic dissemination is the predominant route of ovarian cancer metastasis and a major driver of patient mortality. In our pursuit to identify novel tumor cell adaptations necessary for metastatic spread, we identified a conserved transcriptomic signature activated shortly after matrix-detachment across multiple ascites-derived cell lines and patient specimens.
Read the full summary ↓
Within this signature, RHOV, an atypical and constitutively active Rho GTPase, emerged as the top upregulated gene. We show that RHOV is essential for anoikis resistance, cellular aggregation in anchorage-independence, migration, invasion, in vivo metastatic colonization, and is elevated in patient omental metastases. Mechanistically, RHOV integrates c-Jun dependent cytoskeletal remodeling with early endosomal trafficking to support pro-metastatic signaling, which is dependent on both RHOV’s GTPase activity and membrane localization. For the first time this study identifies RHOV as a necessary mediator of ovarian cancer metastasis, and highlights the potential of immediate early detachment-responsive transcriptional changes as promising therapeutic targets in metastatic disease.
Published in
RHOV Is a Detachment-Responsive Rho GTPase Necessary for Ovarian Cancer Peritoneal Metastasis
Elhaw AT, Tang PW, Cheng YY et al. · Cancer research 2026 · PMID 41989584 · doi:10.1158/0008-5472.CAN-25-4056
This dataset
Open GSE305375 in finder → Find more human RNA-seq datasets → Ovarian cancer landscape →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE305375_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1305427 and SRA study SRP608712. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41989584) ↗
Study design
9 conditions, mostly with about 4 replicates each
OVCA433, Adherent (2D), ×4 OVCA433, Early anchorage-independence (3D-2Hr… ×4 OVCA433, Late anchorage-independence (3D-24Hr… ×4 OVCAR3, Adherent (2D), ×4 OVCAR3, Early anchorage-independence (3D-2Hrs… ×4 OVCAR3, Late anchorage-independence (3D-24Hrs… ×4 SKOV3, Adherent (2D), ×4 SKOV3, Early anchorage-independence (3D-2Hrs), ×4 +1 more

Supports a between-group comparison across 36 samples.

How we read this: 9 replicated groups read from 36 sample titles; they account for 36 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
More Ovarian cancer datasets

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.