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Single-nucleus RNA-seq of peripheral blood mononuclear cells from multiple sclerosis patients and healthy controls

GSE305477 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/08/21 Platform GPL24676
Summary
Multiple sclerosis (MS) is a chronic immune-mediated disease of the central nervous system marked by neuroinflammation and demyelination. Emerging evidence suggests that circulating immune cells contribute to disease activity, heterogeneity of clinical course, and treatment response. Here we provide single-nucleus transcriptomes of peripheral blood mononuclear cells (PBMCs) from individuals with MS and healthy controls. The dataset enables cell-type–resolved interrogation of peripheral immune states associated with MS, including comparisons of cell-state frequencies and gene-expression programs between groups, exploration of inflammatory pathways and ligand–receptor interactions, and discovery of candidate biomarkers. This resource supports integrative analyses with external MS cohorts and facilitates hypothesis generation on mechanisms of immune dysregulation in MS.
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Direct links to NCBI, no account and no request form: the whole study as GSE305477_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1306060 and SRA study SRP608950. Searching any of these in the dataset finder brings you back here.

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