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Post-replicative initial expression of the cell fate regulator PAX6 during neuroectoderm formation [CUT&Tag]

GSE305658 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/09/22 Platform GPL24676
Summary
Human neuroepithelial cells are the earliest neural progenitor cells (NPC) during brain development. The cell cycle is fundamental for cell proliferation and differentiation. How the cell cycle is linked to the differentiation process from embryonic stem cells (ESC) in blastocytes to neuroepithelial cells, however, is still unclear. In this study, using an in vitro ESC-to-NPC differentiation system and PAX6 as an NPC cell fate indicator, we discovered that the neural cell fate was transited, PAX6 expression exhibiting an incremental rise, during the G2 phase. For the mechanism, the loss of -401 bp to -450 bp from the transcription start site (TSS) of PAX6 efficiently blocked the cell cycle-dependent expression of PAX6. In addition, we found that using hydroxyurea to arrest the cell cycle of differentiating NPC could prevent NPC differentiation. Overall, this study reported the first scenario to link cell cycle and cell fate transition during early neurogenesis.
Published in
Post-replicative initial expression of PAX6 during neuroectoderm differentiation
Hu S, Kou R, Su Z et al. · The EMBO journal 2025 · PMID 41120788 · doi:10.1038/s44318-025-00605-y
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Direct links to NCBI, no account and no request form: the whole study as GSE305658_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1307437 and SRA study SRP609621. Searching any of these in the dataset finder brings you back here.

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