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Transcriptomic changes upon doxorubicin treatment in hCMEC/D3

GSE305805 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/11/21 Platform GPL34284
Summary
Current chemotherapy regimens have significantly improved overall survival for children with cancer. However, these curative treatments are associated with detrimental side effects such as chemotherapy-induced cognitive impairment (CICI), or “chemobrain.” Measurable deficits in cognitive function may persist years after chemotherapy treatments, significantly reducing quality of life. Specifically, doxorubicin (DOXO), a commonly used chemotherapeutic agent in curative regimens for children with cancer, plays a pivotal role in the development of CICI, even though it does not cross the blood brain barrier (BBB). We propose to address the poorly understood mechanism of DOXO-related CICI by studying changes induced by DOXO in BBB integrity in vitro, using human cerebral microvascular endothelial cells (hCMEC/D3). Our findings provide critical insights on how DOXO impacts the BBB and builds a foundation for developing preventative measures and therapeutic interventions that may improve the quality of life for patients.
Published in
The effects of doxorubicin on blood-brain barrier integrity in hCMEC/D3
Patel C, Glytsou C, Jang MH et al. · Neurotoxicology 2025 · PMID 41265652 · doi:10.1016/j.neuro.2025.103355
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Direct links to NCBI, no account and no request form: the whole study as GSE305805_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1307863 and SRA study SRP610066. Searching any of these in the dataset finder brings you back here.

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