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Effect of Aiolos on virtual memory T cell differentiation and function [ATAC-Seq]

GSE305887 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2025/11/10 Platform GPL34328
Summary
Virtual memory CD8+ T (TVM) cells provide rapid bystander protection during an infection despite their antigen naivety. In addition, these cells are also capable of antigen-specific response, making them an intriguing part of both the host innate and adaptive immune repertoire. While the innate and adaptive capabilities of these cells have been well characterized, much remains to be defined regarding the mechanisms governing their differentiation and function. Here, we investigate the role of the Ikaros zinc-finger (IkZF) transcription factor Aiolos in regulating CD8+ TVM generation and function. Using bulk RNA-sequencing and Assay for Transposase-Accessible Chromatin (ATAC)-sequencing, we identify differences in gene expression as well as accessibility profiles of key TVM effectors and regulators in TVM cells isolated from the spleen of control (wild-type) and Aiolos-deficient (Ikzf3-/-) mice. These studies will provide mechanistic insight into the regulation of TVM differentiation and function by Aiolos.
Published in
Aiolos restricts the generation of antigen-inexperienced, virtual memory CD8(+) T cells in mice
Pokhrel S, Dileepan G, Leonard MR et al. · Nature communications 2025 · PMID 41392082 · doi:10.1038/s41467-025-67540-8
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Direct links to NCBI, no account and no request form: the whole study as GSE305887_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1308121 and SRA study SRP610057. Searching any of these in the dataset finder brings you back here.

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