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RNA-seq of Epithelial cells treated with siRNA and irradiation or cytokine treatment

GSE306184 Homo sapiens (human) RNA-seq (gene expression) 14 samples Submitted 2025/09/02 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 14 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
14 samples: 6 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
N-phenylquinazolin-4-amine-based EGFR TKIs suppress pulmonary fibrosis by modulating the EGFR/ERBB3 axis in epithelial-macrophage interactionCommunications biology · 2025
Summary (written by the submitters)
RNA-seq of lung epithelial cells with EGFR or ErbB deletion, harvested 3 days after irradiation or cytokine treatment. RNA was extracted, libraries were prepared using a stranded RNA-seq protocol, and sequencing was performed on the Illumina platform.
Published in
N-phenylquinazolin-4-amine-based EGFR TKIs suppress pulmonary fibrosis by modulating the EGFR/ERBB3 axis in epithelial-macrophage interaction
Kim JH, Choi SH, Nam JK et al. · Communications biology 2025 · PMID 41326761 · doi:10.1038/s42003-025-08940-w
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE306184_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1309651 and SRA study SRP610730. Searching any of these in the dataset finder brings you back here.

Study design
6 conditions, mostly in duplicate
RNA seq of Epithelial cells treated with EGFR… ×2 RNA seq of Epithelial cells treated with EGFR… ×2 RNA seq of Epithelial cells treated with Erbb… ×2 RNA seq of Epithelial cells treated with cont… ×2 RNA seq of Epithelial cells treated with cont… ×2 RNA seq of Epithelial cells treated with cont… ×2 RNA seq of Epithelial celsl treated with Erbb… ×1 RNA seq of Epithelial cells treated with Erbb… ×1

Supports a between-group comparison across 12 samples.

How we read this: 6 replicated groups read from 14 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.