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Explore the molecular profile and properties of quiescent (P27+) and mitotic (P27-) glioma cell in diffuse low grade astrocytoma cell lines LGG275

GSE306414 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/01/13 Platform GPL29480
Summary
Astrocytomas are known to present a cellular heterogeneity consisting of three identified subpopulations: "astrocyte-like", "oligodendrocyte-like" and "NPC-like" cells. It has also been shown that they contain a subpopulations of quiescent, or neural G0 cells, representing more than 90% of the tumor cells. The properties of this cell remain unclear due to the fact that it is difficult to isolate them. We used mVenus/P27 vector expression, constructed by Oki et al (2014), where P27 is fused to mVenus to highlight quiescent cells in LGG275 cell lines, a slow-growing astrocytoma. Then we used this reporter system to sort the mVenus/P27 expressing cells (quiescent) and the negative cells (mitotic) in aims to explore the properties of these two populations and validate the mVenus/P27 tool.
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Also filed as BioProject PRJNA1310597 and SRA study SRP612346. Searching any of these in the dataset finder brings you back here.

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