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Rejuvenation of mesenchymal stem cells by human peripheral blood lymphocytes

GSE307003 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/09/02 Platform GPL24676
Summary
Here we presented a novel approach to combat cellular senescence and promote cell proliferation by coculturing human MSCs with human peripheral blood lymphocytes (PBLs). In a heterogeneous population of MSCs comprising both aged and nonaged cells, PBL effector cells, rather than their cytokines, exhibited a dual role. They selectively induced apoptosis in aged cells by facilitating p53 SUMOylation and activating the p53-dependent mitochondrial pathway, while simultaneously safeguarding younger cells against senescence and promoting cell proliferation by activating Serpinb2/NF-κB signaling. This resulted in a decrease in aged MSCs and an enrichment of rejuvenated MSCs. This process effectively reversed the senescence phenotype, leading to the remodeling of stemness characteristics and the enhancement of functionality within the MSC population. Furthermore, MSCs rejuvenated by PBLs presented an enhanced therapeutic efficacy and a favorable safety profile.
Published in
Rejuvenation of mesenchymal stem cells by human peripheral blood lymphocytes
Luo Y, Zhu XX, Le QR et al. · BMC biology 2025 · PMID 41291793 · doi:10.1186/s12915-025-02472-9
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Also filed as BioProject PRJNA1314089 and SRA study SRP616480. Searching any of these in the dataset finder brings you back here.

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