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Microglia-containing neural organoids as brain microphysiological systems for long-term culture

GSE307124 Homo sapiens (human) RNA-seq (gene expression) 3 samples Submitted 2025/09/10 Platform GPL34281
At a glance

RNA-seq (gene expression) · human · 3 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
3 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Microglia-containing neural organoids as brain microphysiological systems for long-term cultureFrontiers in cellular neuroscience · 2025
Summary (written by the submitters)
Microglia are cells essential for many aspects of brain development, homeostasis, and neuroinflammation that originate from the yolk sac during embryogenesis and migrate to the developing brain. Considering their developmental origin, many brain organoid models would naturally lack them and require a co-culture. We developed a microglia-integrated brain organoid model (immune-competent brain microphysiological system: µbMPS) using hiPSC-derived neural and microglia progenitors aggregated in U-bottom 96-well plates, enabling controlled and reproducible incorporation of microglia progenitors.
Read the full summary ↓
We demonstrated that microglia integrated, matured, and survived long-term in the neural environment without costly exogenous microglia-specific growth factors and cytokines. We maintained microglia for over nine weeks, demonstrating functional activity, phagocytosis, and neuroinflammatory responses. µbMPS exhibited enhanced neuronal activity and maturity, offering a scalable, reproducible model for neurodevelopment, disease modeling, and neurotoxicology research.
Published in
Microglia-containing neural organoids as brain microphysiological systems for long-term culture
Rittenhouse A, Krall C, Plotkin J et al. · Frontiers in cellular neuroscience 2025 · PMID 41114125 · doi:10.3389/fncel.2025.1616470
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE307124_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1314622 and SRA study SRP616823. Searching any of these in the dataset finder brings you back here.

Samples in this study

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.