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hnRNPC engages mature RNAs during metaphase: RNA-seq and fCLIP in proTAME-arrested HeLa/HeLa S3 cells

GSE307226 Homo sapiens Expression profiling by high throughput sequencing; Other 6 samples Submitted 2026/04/12 Platform GPL30173Platform GPL24676
Summary
Proper progression through mitosis requires coordinated changes in RNA metabolism, but the specific contributions of individual RNA-binding proteins remain unclear. Heterogeneous nuclear ribonucleoprotein C (hnRNPC) is a ubiquitous RBP that normally binds uridine-rich sequences to influence pre-mRNA processing and stability. Here we investigated the role of hnRNPC in mitotic cells by profiling both global RNA abundance and hnRNPC–RNA interactions during metaphase arrest. To this end, we combined bulk RNA-seq with fluorescent crosslinking and immunoprecipitation (fCLIP) in proTAME-arrested HeLa S3 cells. This dataset enables the characterization of hnRNPC binding to mature RNAs during mitosis, the evaluation of biochemical fractionation (low-density vs high-density complexes), and the assessment of binding site resolution across distinct RNase digestion conditions. Together, the study provides a resource for exploring how hnRNPC contributes to RNA stability and gene expression control during cell division
Published in
Repurposed hnRNPC binds mature mRNAs and safeguards the mitotic transcriptome
Lev-Ari L, Laster S, Atzmon A et al. · Nucleic acids research 2026 · PMID 42049236 · doi:10.1093/nar/gkag391
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Direct links to NCBI, no account and no request form: the whole study as GSE307226_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1314792 and SRA study SRP617314. Searching any of these in the dataset finder brings you back here.

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