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Base pair resolution chromosome conformation capture reveals mechanistic insights into the nature of chromatin structure

GSE307228 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 5 samples Submitted 2025/11/26 Platform GPL24247
Summary
Chromatin structure is a key determinant of gene expression in eukaryotes but it has not previously been possible to define the full structure of regulatory elements at the scale of the proteins determining gene expression. Here we generate multidimensional chromosome conformation capture (3C) maps at single base-pair resolution using Micro Capture-C (MCC). This allows contacts between individual transcription factor binding sites within regulatory elements to be defined. Using degrons-we investigate the structural role of transcription factors and the Mediator complex. We show that the biophysical properties of nucleosomes and DNA can replicate the nanoscale chromatin structures observed with MCC using a detailed chemically specific, molecular dynamics approach. The ultra-high resolution 3C enable molecular dynamics approaches to be linked to larger scale nuclear architecture defined by super-resolution microscopy and allow us to propose a unified model to describe chromatin structure based on these orthogonal approaches.
Published in
Mapping chromatin structure at base-pair resolution unveils a unified model of cis-regulatory element interactions
Li H, Dalgleish JLT, Lister G et al. · Cell 2025 · PMID 41197626 · doi:10.1016/j.cell.2025.10.013
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Also filed as BioProject PRJNA1314791 and SRA study SRP617401. Searching any of these in the dataset finder brings you back here.

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