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Forced intensity-controlled endurance training on a small-animal treadmill machine inducing murine cardiac hypertrophy

GSE308626 Mus musculus (mouse) RNA-seq (gene expression) 10 samples Submitted 2025/12/02 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 10 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
10 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Forced intensity-controlled endurance training on a small-animal treadmill machine inducing murine cardiac hypertrophy: insights and comparison to voluntary running modelsFrontiers in pharmacology · 2025
Summary (written by the submitters)
Endurance training is associated with decreased cardiovascular-related morbidity and mortality. Cardiac hypertrophy is an adaptive mechanism of the heart in response to volume and pressure overload. Nonetheless, murine exercise models for cardiac hypertrophy are still under discussion. Using a small-animal treadmill, a forced intensity controlled training model was conducted to characterize cardiac hypertrophy in mice utilizing multimodal analyses.
Read the full summary ↓
Wild-type male C57BL/6 mice age between 5-8 weeks old were subjected to 8 weeks of forced endurance training using a small-animal treadmill or sedentary age-matched control. Transcriptomic analysis of 8-week training hearts was evaluated using RNA sequencing. RNA sequencing revealed several genes involved in the cell cycle, apoptosis, contractile protein expression, and organ growth that were among the most differentially regulated genes after 8-weeks of exercise.
Published in
Forced intensity-controlled endurance training on a small-animal treadmill machine inducing murine cardiac hypertrophy: insights and comparison to voluntary running models
Fischer M, Simahendra A, Straub T et al. · Frontiers in pharmacology 2025 · PMID 41142228 · doi:10.3389/fphar.2025.1682751
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE308626_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1331740 and SRA study SRP622533. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 10 more — browse all 10 samples with per-sample file links →

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