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Single-cell RNA-seq of undirected human cerebral organoids (HuCOs) infected with MV1 or MV2 compared with uninfected controls at 120 and 180 days post-infection

GSE309815 Homo sapiens Expression profiling by high throughput sequencing 7 samples Submitted 2025/11/18 Platform GPL24676
Summary
Undirected human cerebral organoids (HuCOs) derived from induced pluripotent stem cells (hiPSCs) were used to model neural development and infection response in vitro. Organoids were maintained in spinning bioreactors and divided into three experimental groups: MV1-infected, MV2-infected, and uninfected control (NBH). Each infection group was harvested at two developmental stages, approximately 120 and 180 days post-infection (dpi), following five months of infection. Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 and sequenced on an Illumina NovaSeq 6000 platform. The study aimed to characterize infection-induced transcriptional and cellular changes across time points and between infection subtypes. This dataset provides a high-resolution single-cell transcriptomic resource to explore cellular heterogeneity and host–pathogen interactions in undirected human cerebral organoids.
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Direct links to NCBI, no account and no request form: the whole study as GSE309815_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1363758 and SRA study SRP644870. Searching any of these in the dataset finder brings you back here.

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