Regulation of murine follicle-stimulating hormone β subunit transcription by newly identified enhancers

Activin-class ligands of the TGFβ family stimulate follicle-stimulating hormone (FSH) synthesis in pituitary gonadotrope cells through SMAD3/SMAD4 and FOXL2-mediated transcriptional regulation of Fshb. This SuperSeries integrates chromatin accessibility and transcription factor binding datasets to identify activin-sensitive regulatory regions controlling Fshb expression. It includes single-nucleus ATAC-seq of mouse pituitaries, and bulk ATAC-seq and ChIP-seq of gonadotrope-derived LβT2 cells. Together, these datasets indicate that there may be as many as four activin-sensitive enhancers upstream of murine Fshb.